Statistical optimization of medium for the production of recombinant hirudin from Saccharomyces cerevisiae using response surface methodology

摘要: Abstract An efficient fermentation medium producing a colourless product with high yields has been developed for the production of recombinant hirudin from Saccharomyces cerevisiae . Response surface methodology was applied to optimize constituents. A 2 5-1 fractional factorial central composite design chosen explain combined effect six constituents, viz. yeast extract, peptone, casamino acids, ammonium sulphate, potassium phosphate and galactose, minimum number experiments. The optimized produced 65.3 mg/l r-hirudin in shake flask culture, which is 35% higher than unoptimized medium. colour purified influenced by intensity culture supernatant, turn depended upon concentration complex substrates used extract reduced 16 g/l other reported media containing 40 extract. supernatant obtained 60%