Production of hirudin by recombinant Saccharomyces cerevisiae in a membrane-recycle fermentor

摘要: Recombinant Saccharomyces cerevisiae was employed to continuously produce hirudin in a membrane cell recycle fermentor. The gene cooing for the anticoagulant protein combined with GAL10 promoter controlled expression and MF α 1 signal sequence secretion fermentation broth. A dilution rate of 0.1h−1 yielded maximum concentration 59mg / l specific 2.4 mg /g mass among rates studied ranging from 0.05h−1 0.3h−1. Cell bleeding gave same results as without bleeding. productivity process 6.0mg hirudin/l · hr, corresponding 1.7-fold increase compared conventional continuous culture.