Continuous production of cell-free recombinant proteins using Escherichia coli

摘要: Continuous production of extracellular recombinant protein using a previously reported periplasmic protein-release system was successfully demonstrated. The performance the protein-releasing studied two types reactor configuration: conventional chemostat and with cell-recycle system. constitutively expressed alpha-amylase used as model protein. In experiments, total activity increases decreasing dilution rate--from 135.5 units/mL at rate 0.05 h-1 to 2.06 0.491 h-1. However, percentage release remained relatively constant, approximately 29%, in range rates investigated. productivity target reached maximum 8.14 units/mL/h 0.1 Application releasing for continuous cell-free proteins fermentor also studied. is significantly better than that either or batch mode. A 13.2 42% recovery form obtained 0.397 bleeding 9.8%.