Bioactive Natural Protein-Hydroxyapatite Nanocarriers for Optimizing Osteogenic Differentiation of Mesenchymal Stem Cells.
作者： Z. Z. Ding , Z. H. Fan , X. W. Huang , S. M. Bai , D. W. Song
DOI: 10.1039/C6TB00509H
关键词:
摘要: Improving the controlled release of bioactive growth factors to regulate cell behavior and tissue regeneration remains a need in engineering regenerative medicine. Inorganic polymeric nanoparticles have been extensively fabricated as biomaterials with enhanced biocompatibility effective carriers therapeutic agents, however, challenges remain such achievement high loading capacity sustained release, bioactivity preservation factors. Here, multilayered, silk coated hydroxyapatite (HA) nanocarrier drug loading-release superior pure or HA was developed. Bone morphogenetic protein-2 (BMP-2) bound coatings binding efficiency 99.6%, significantly higher than that alone. The BMP-2 vitro over period 21 days without burst release. Compared loaded particles, bone mesenchymal stem cells (BMSCs) showed improved proliferation osteogenesis when cultured composite nanocarriers. Therefore, these silk–HA are useful designing systems functions for needs.
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