A cell-compatible PEO-PPO-PEO (Pluronic®)-based hydrogel stabilized through secondary structures.
作者： Sydney Peng , Ji-Yu Lin , Ming-Huei Cheng , Chih-Wei Wu , I-Ming Chu
DOI: 10.1016/J.MSEC.2016.06.091
关键词:
摘要: Abstract Pluronic F-127 (PF127) is a thermosensitive polymer that has been widely recognized as potential candidate for various bio-applications. However, in hydrogel form, its rapid disintegration and inhospitality toward cells have significantly limited usage. As means to increase the integrity cell compatibility of PF127 hydrogel, we propose introduction stabilizing secondary structures gel network by addition structure-forming oligo-alanine oligo-phenylalanine. Results indicate increasing oligo(peptides) attached led significant decrease gelation concentration temperature. A selected oligo(peptide)-modified was capable forming stable at 5% suffered only 20% weight loss after 7 days incubation media. Scanning electron microscopy (SEM) revealed comparably more interconnected morphology modified hydrogels which may be attributed presence structures, verified circular dichroism (CD) Fourier-transformed infrared (FT-IR) spectroscopy. Nuclear magnetic resonance (NMR) provided insights into extensive interactions micelle core, key altered behavior. Furthermore, maintained structural within culturing media supported proliferation encapsulated chondrocytes. In addition, vivo residence time extended well beyond 2 weeks oligo(peptide) modification, thereby broadening application scope encompass long-term drug delivery culturing.
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