The chemistry of the collagen cross-links. Characterization of the products of reduction of skin, tendon and bone with sodium cyanoborohydride.

摘要: Reduction of tissues with sodium cyanoborohydride at pH7.4 gave results identical those obtained by KBH4 treatment. On reduction pH 4.4, however, a previously undetected basic compound was formed and identified mass spectrometry chemical degradation techniques as dihydrohydroxymerodesmosine. Histidino-hydroxymerodesmosine not present, further analysis confirmed that reduced aldol, mojor product the lower pH, also absent. These results, together an time course reduction, support previous assertions histidino-hydroxymerodesmosine is artifact [robins *Bailey (1973) Biochem. J. 135, 657-665] suggests non-reduced form hydroxymerodesmosine probably does constitute major intermolecular bond in vivo.