Keratoconus: I. Biochemical studies
作者： James W. Critchfield , Anthony J. Calandra , Anthony B. Nesburn , M. Cristina Kenney
DOI: 10.1016/S0014-4835(88)80047-2
关键词: Chemistry 、 Lectin 、 Cornea 、 Anatomy 、 Guanidine 、 Keratoconus 、 Western blot 、 Agglutinin 、 Uronic acid 、 Molecular biology 、 Hydroxylysine
摘要: The present study analyses collagenous and non-collagenous components from age-matched normal keratoconus corneas. Intact corneas showed decreased collagen, total protein, hydroxylysine levels, with reducible collagen cross-linking. Non-collagenous fractions were isolated a 4 m guanidine procedure. As demonstrated by PAGE-silver stain, the cornea extracts had 75 kDa band that was absent in extracts. In addition, there markedly increased level of uronic acid neutral hexose as compared controls. Our Western blot studies affinity for castor-bean agglutinin (RCA 120 , specific terminal galactose) normals. These data suggest presence an abnormal noncollagenous component
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