Localization of lectin binding sites in human, cat, and rabbit corneas.

摘要: Paraffin sections of human, cat, and rabbit corneas were stained with nine lectins, using an avidin-biotin-complex procedure to study glycoconjugates the epithelium, keratocytes, stromal matrix. Wheat germ agglutinin (WGA) plasma membranes all epithelial cell layers cat human superficial wing cells rabbit. Plasma epithelium also peanut (PNA) Ricinus communis I (RCA-I). Human keratocytes WGA RCA-I. Stromal matrices three species concanavalin A lentil agglutinin. In neuraminidase-treated sections, entire PNA. Corneal from did not stain Bandeiraea simplicifolia I, II, Ulex europeus Soybean These data suggest presence oligosaccharides with: N-acetylglucosamine/sialic acid residues in corneal studied cat; terminal beta-galactose beta-galactose-galactosamine chains membranes; sialic acid-beta-galactose-galactosamine species.