Binding of encephalomyocarditis virus RNA polymerase to the 3'-noncoding region of the viral RNA is specific and requires the 3'-poly(A) tail.
作者： T Cui , S Sankar , A G Porter
DOI: 10.1016/S0021-9258(19)74285-9
关键词:
摘要: An in vitro RNA bandshift assay has been developed to demonstrate the binding of purified recombinant encephalomyocarditis (EMC) virus RNA-dependent polymerase (3Dpol) 3'-noncoding region (NCR) and 30 nucleotides adjacent 3'-terminal poly(A) tail (3'-NCR(A)) EMC RNA. The 3Dpol 3'-NCR(A) fragment was specific since four other unrelated proteins including an did not bind, unlabeled 3'-NCR(A), but alpha-globin mRNA, tRNA, or pure poly(A), competed with radiolabeled for binding. Surprisingly, failed bind 3'-NCR lacking tail. results together show that template specificity depends only on 3Dpol, 3'-NCR, This suggests 3'-poly(A) is essential viral selection by polymerase.
sci-hub.st 参考文章(39)
O.C. Richards, P Yu, K.L. Neufeld, E Ehrenfeld, Nucleotide binding by the poliovirus RNA polymerase. Journal of Biological Chemistry. ,vol. 267, pp. 17141- 17146 ,(1992) , 10.1016/S0021-9258(18)41905-9
S Sankar, A G Porter, Expression, purification, and properties of recombinant encephalomyocarditis virus RNA-dependent RNA polymerase Journal of Virology. ,vol. 65, pp. 2993- 3000 ,(1991) , 10.1128/JVI.65.6.2993-3000.1991
Yoshiaki Yogo, Eckard Wimmer, Sequence studies of poliovirus RNA Journal of Molecular Biology. ,vol. 92, pp. 467- 477 ,(1975) , 10.1016/0022-2836(75)90292-2
M H Baron, D Baltimore, Purification and properties of a host cell protein required for poliovirus replication in vitro. Journal of Biological Chemistry. ,vol. 257, pp. 12351- 12358 ,(1982) , 10.1016/S0021-9258(18)33720-7
Deborah M. Tuschall, Ernest Hiebert, James B. Flanegan, Poliovirus RNA-Dependent RNA Polymerase Synthesizes Full-Length Copies of Poliovirion RNA, Cellular mRNA, and Several Plant Virus RNAs In Vitro Journal of Virology. ,vol. 44, pp. 209- 216 ,(1982) , 10.1128/JVI.44.1.209-216.1982
K Dorsch-Häsler, Y Yogo, E Wimmer, Replication of picornaviruses. I. Evidence from in vitro RNA synthesis that poly(A) of the poliovirus genome is genetically coded. Journal of Virology. ,vol. 16, pp. 1512- 1517 ,(1975) , 10.1128/JVI.16.6.1512-1517.1975
T D Hey, O C Richards, E Ehrenfeld, Host factor-induced template modification during synthesis of poliovirus RNA in vitro. Journal of Virology. ,vol. 61, pp. 802- 811 ,(1987) , 10.1128/JVI.61.3.802-811.1987
Deborah H. Spector, David Baltimore, Polyadenylic acid on poliovirus RNA IV. Poly(U) in replicative intermediate and double-stranded RNA. Virology. ,vol. 67, pp. 498- 505 ,(1975) , 10.1016/0042-6822(75)90450-X
T A Van Dyke, R J Rickles, J B Flanegan, Genome-length copies of poliovirion RNA are synthesized in vitro by the poliovirus RNA-dependent RNA polymerase. Journal of Biological Chemistry. ,vol. 257, pp. 4610- 4617 ,(1982) , 10.1016/S0021-9258(18)34768-9
N Takeda, R J Kuhn, C F Yang, T Takegami, E Wimmer, Initiation of poliovirus plus-strand RNA synthesis in a membrane complex of infected HeLa cells. Journal of Virology. ,vol. 60, pp. 43- 53 ,(1986) , 10.1128/JVI.60.1.43-53.1986