Freeze-tolerance of Trichinella muscle larvae in experimentally infected wild boars.

摘要: Freeze-tolerance of encapsulated Trichinella muscle larvae (ML) is mainly determined by species, but also influenced host the age infection and storage time temperature infected meat. Moreover, freeze-tolerance species appears to be correlated development thick capsule walls which increases with age. An extended period composition in some hosts (e.g. herbivores) may provide freeze-avoiding matrices due high carbohydrate contents. The present experiment compares spiralis britovi ML wild boar meat 24 weeks post inoculation (wpi). Three groups four boars were 200, 2000 or 20,000 T. (ISS 1575), respectively. Additionally, three inoculated 004) two animals served as negative controls. All sacrificed wpi. Muscle samples 70 g stored at -21°C for 19, 30 56 h, 1-8 weeks. Larvae recovered artificial digestion. Their mobilities recorded using Saisam(®) image analysis software their infectivities evaluated mouse bioassays. Samples frozen h allowed recovery mobile ML, 1 2 did not. Correspondingly, only isolated from established mice. This study showed that freezing week inactivated