Targeting melanocyte and melanoma stem cells by 8-hydroxy-2-dipropylaminotetralin.

摘要: Monobenzyl ether of hydroquinone (MBEH) is cytotoxic towards melanocytes. Its treatment efficacy limited by an inability to eradicate stem cells. By contrast, 8-hydroxy-N,N-dipropyl-2-aminotetralin (8-DPAT) affects melanocyte cell survival. MBEH and 8-DPAT were added melanocytes melanoma cells compare cytotoxicity. Stem content among viable was determined fluorocytometry using markers CD34, Pax3, CD271. Immunostaining used identify in skin explants treated with or ex vivo. Mice exposed scanned for depigmentation before harvesting skin. exposure prompted a relative increase cultured cells, as preferentially eliminated differentiated spared the Viability this remaining, enriched population however rapidly reduced within cultures. In human explants, abundance also visibly after treatment, contrast tissue MBEH. Meanwhile, significant mouse pelage loss observed vivo response topical application MBEH, but not 8-DPAT. Prolonged latter agent instead appeared effectively reduce dermis. This furthers idea that target complementary populations. Results indicate combination may demonstrate superior therapeutic activity eliminating both tumor initiating