Nucleic acid process containing improved molecular switch

作者: Paul M. Lizardi , Hilda M. L. Buyoli , Sanjay Tyagi , Cesar E. Guerra , Fred R. Kramer

DOI:

关键词:

摘要: A probe for the detection of a nucleic acid target sequence containing molecular switch comprising three essential elements: 20-60 nucleotides surrounded by sequences 10-40 which are complementary to each other, wherein state is useful selectively generating detectable signal if hybridized target; also, assays and kits utilizing such probes.

参考文章(9)
F. R. Kramer, P. M. Lizardi, Replicatable RNA reporters Nature. ,vol. 339, pp. 401- 402 ,(1989) , 10.1038/339401A0
P. R. Langer, A. A. Waldrop, D. C. Ward, Enzymatic synthesis of biotin-labeled polynucleotides: novel nucleic acid affinity probes Proceedings of the National Academy of Sciences of the United States of America. ,vol. 78, pp. 6633- 6637 ,(1981) , 10.1073/PNAS.78.11.6633
Stephen A. Krawetz, Bernd W. Kalisch, Johan H. van de Sande, Covalently linked complementary oligodeoxynucleotides (splinkers) as tools for molecular biology. Nucleic Acids Research. ,vol. 14, pp. 7131- 7131 ,(1986) , 10.1093/NAR/14.17.7131
T. NISHIHARA, D. R. MILLS, F. R. KRAMER, Localization of the Qβ Replicase Recognition Site in MDV-1 RNA Journal of Biochemistry. ,vol. 93, pp. 669- 674 ,(1983) , 10.1093/JB/93.3.669
Bernd W. Kalisch, Stephen A. Krawetz, Karl-Heinz Schoenwaelder, Johan H. van de Sande, Covalently linked sequencing primer linkers (splinkers) for sequence analysis of restriction fragments. Gene. ,vol. 44, pp. 263- 270 ,(1986) , 10.1016/0378-1119(86)90190-3
D. R. Engelke, P. A. Hoener, F. S. Collins, Direct sequencing of enzymatically amplified human genomic DNA. Proceedings of the National Academy of Sciences of the United States of America. ,vol. 85, pp. 544- 548 ,(1988) , 10.1073/PNAS.85.2.544
Herman A. DeBoer, Microbial hybrid promoters ,(1982)