Assessment of the in vitro and in vivo activity of atorvastatin against Candida albicans.

摘要: Aim. The aim of this work was to characterize the response Candida albicans atorvastatin, and assess its in vivo antifungal capability. Methodology. effect atorvastatin on growth viability C. assessed. ability statin alter cell permeability quantified by measuring amino acid protein leakage. assessed using label-free quantitative proteomics. activity Galleria mellonella larvae infected with albicans. Results. Atorvastatin inhibited atorvastatin-treated cells showed lower ergosterol levels than controls, demonstrated increased calcofluor staining released elevated quantities acids protein. Larvae a survival rate 18.1±4.2 % at 144 h. In contrast, administered (9.09 mg kg−1) displayed 60.2±6.4 (P<0.05). Label-free proteomics identified 1575 proteins 2 or more peptides 465 were differentially abundant There an increase abundance enzymes oxidoreductase hydrolase cells, squalene monooxygenase (4.52-fold increase) lanosterol synthase (2.84-fold abundance. Proteins such as small heat shock 21 (−6.33-fold) glutathione peroxidase (−2.05-fold) reduced Conclusion. results presented here indicate that inhibits is capable increasing G.