Complexes containing cationic and anionic pH-sensitive liposomes: comparative study of factors influencing plasmid DNA gene delivery to tumors.

摘要: pH-sensitive liposomes represent an effective gene vector in cancer therapy. However, their use is greatly hampered by relatively low transfection efficiency. To improve the efficiency of liposomes, we prepared complexes containing 3β-[N-(N′,N′-dimethylaminoethane) carbamoyl] cholesterol (DC-Chol) and dioleoylphosphatidyl ethanolamine (DOPE) composed cholesteryl hemisuccinate (CHEMS) DOPE, evaluated influence various factors on plasmid DNA (pDNA) All DC-Chol/DOPE liposome/pDNA liposome showed similarly potent pH sensitivity. In presence serum-containing medium, two optimized liposomes/pDNA PEGylated high 22.94% 20.07%, respectively. Notably, (2:3) liposomes/pH-sensitive (1%) with a charge ratio 1:1 (m/m [+/−]) enhanced accumulation tumors vivo. Our results show pDNA liposomes. Understanding such mechanisms will lead to better design for