Methionine synthesis from 5'-methylthioadenosine in rat liver.
作者： Peter S Backlund , Roberts A Smith
DOI: 10.1007/978-1-349-06343-7_102
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摘要: A pathway resulting in the formation of methionine from 5'-methylthioadenosine is shown vitro using cell-free homogenates rat liver. Under conditions used, was major product produced, as determined by its chemical and chromatographic properties. The kinetics indicated that first rapidly converted to 5-methylthioribose 1-phosphate, followed slower conversion methionine. 5'-[Methyl-14C]methylthioadenosine, 5'-[methyl-3H]-methylthioadenosine, 5'-[35S]methylthioadenosine, 5'-[adenosine-U-14C]methylthioadenosine were synthesized determine which portion molecule became incorporated into Carbons ribose portion, carbon hydrogens methyl group, sulfur are all ratio incorporation 1:1. Therefore, for synthesis involves modifying 2-aminobutyrate methionine, with thiomethyl group remaining intact. This appears be a significant salvage mammals, may necessary removal produced by-product polyamine biosynthesis.
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