Drug resistance testing of acute myeloid leukemia in adults using the MTT assay.

摘要: Objective: We want to evaluate the MTT in vitro assay for newly diagnosed AML adults, particular its prognostic significance. Methods: tests were performed according Pieters et al., Blood 76: 2327, 1990. Up 18 cytostatic drugs tested a wide range of concentrations over 4 days and compared with controls. If necessary, blasts enriched > 80 % by negative selection dynabeads (Kaspers Br. J. Cancer 70: 1047, 1994). Percent S-phase was measured at begin after days. Results: Technical success rate 80–85 took about one day work. IC50 values as measure drug resistance highly reproducible an interassay CV (range) 28 (13–47) between Median cell viability controls (44–97); 3 (0.1–15) 9 (1–39). Leukemic from 57 adult patients prospectively diagnosis. Large interindividual differences observed (over 1000 fold on average). There trend greater higher cytogenetic risk nonresponders induction. Other clinical factors such patient age, initial WBC, FAB subtype, prior MDS did not show relationship data. high correlation topoisomerase II related regarding resistance. Conclusion: In sensitivity like may provide valuable tool prediction individual therapy outcome combination cytogenetics. However, longer follow-up is required.