On the structure of the hemocyanin channel in lipid bilayers.
作者： T.J. McIntosh , J.D. Robertson , H.P. Ting-Beall , A. Walter , G. Zampighi
DOI: 10.1016/0005-2736(80)90534-9
关键词:
摘要: Abstract Keyhole limpet hemocyanin has been shown by others (Alvarez, O., Diaz, E. and Latorre, R. (1975) Biochim. Biophys. Acta 389, 444–448) to form single conductance channels in black lipid membranes. In an attempt visualize how the large (300 A) water-soluble molecule interacts with bilayers, we have examined presence of lipids electron microscope. We find that incubation keyhole produces a characteristic 70 A diameter, ring-shaped particle or annulus associated bilayer. This annulus, which appears be quite distinct from previously observed aggregated dissociated forms hemocyanin, may responsible for channel formation
elsevier.com
sci-hub.se 参考文章(26)
T.J. McIntosh, R.C. Waldbillig, J.D. Robertson, Lipid bilayer ultrastructure Biochimica et Biophysica Acta (BBA) - Biomembranes. ,vol. 448, pp. 15- 33 ,(1976) , 10.1016/0005-2736(76)90073-0
E.F.J. van Bruggen, E.H. Wiebenga, M. Gruber, Structure and properties of hemocyanins. II. Electron micrographs of the hemocyanins of Sepia officinalis, Octopus vulgaris and Cancer pagurus. Journal of Molecular Biology. ,vol. 4, pp. 8- 9 ,(1962) , 10.1016/S0022-2836(62)80111-9
H. Fernández-Morán, E.F.J. van Bruggen, M. Ohtsuki, Macromolecular organization of hemocyanins and apohemocyanins as revealed by electron microscopy. Journal of Molecular Biology. ,vol. 16, pp. 191- 207 ,(1966) , 10.1016/S0022-2836(66)80272-3
J. E. MELLEMA, A. KLUG, Quaternary Structure of Gastropod Haemocyanin Nature. ,vol. 239, pp. 146- 150 ,(1972) , 10.1038/239146A0
R. Antolini, G. Menestrina, Ion conductivity of the open keyhole limpet hemocyanin channel FEBS Letters. ,vol. 100, pp. 377- 381 ,(1979) , 10.1016/0014-5793(79)80374-9
P. N. T. Unwin, G. Zampighi, Structure of the junction between communicating cells Nature. ,vol. 283, pp. 545- 549 ,(1980) , 10.1038/283545A0
W. J. HARRIS, Holey Films for Electron Microscopy Nature. ,vol. 196, pp. 499- 500 ,(1962) , 10.1038/196499B0
E.F.J. van Bruggen, H. Fernández-Morán, Re-association of hemocyanins from subunit mixtures. Journal of Molecular Biology. ,vol. 16, pp. 208- ,(1966) , 10.1016/S0022-2836(66)80273-5
R.C. Waldbillig, J.D. Robertson, T.J. McIntosh, Images of divalent cations in unstained symmetric and asymmetric lipid bilayers Biochimica et Biophysica Acta (BBA) - Biomembranes. ,vol. 448, pp. 1- 14 ,(1976) , 10.1016/0005-2736(76)90072-9
R. Latorre, O. Alvarez, G. Ehrenstein, M. Espinoza, J. Reyes, The nature of the voltage-dependent conductance of the hemocyanin channel. The Journal of Membrane Biology. ,vol. 25, pp. 163- 181 ,(1975) , 10.1007/BF01868573