Assessment of the Proliferative Marker Ki-67 and p53 Protein Expression in HBV- and HCV-related Hepatocellular Carcinoma Cases in Egypt.

摘要: Background: Chronic HBV and HCV infections are the major risk factors for development of HCC through a multistep pathway that involves viral non-viral dependent pathophysiological steps. Hepatic expression nuclear proliferative marker ki-67 p53 oncoprotein were found to be associated with poor outcome. So, present study was done evaluate changes in Ki-67 oncoprotein, determine gene mutation HBV/HCV-related Egyptian patients. Methods: Eight HBV-and 22 HCV-positive cases have been examined presence by immunohistochemistry (IHC) single-strand conformation polymorphism (SSCP), followed direct DNA sequencing. genotyped LiPA-II. Results: Our results shown LI highly expressed signicantly related tumor grade (p<0.05). Also, 16 IHC 14 SSCP, only 11 patients showed The highest rate scored exon 7 (7 mutations) at codon 249; 4 out 8 (50%) HBV-related 3 (13.6%) HCV-related cases, 5 (3 codons 133, 146, 176 then 275 cases. concordance between sequencing analysis 69%. Conclusion: demonstrates association also support hypothesis mutations rather late event carcinogenesis. they suggest nal steps hepatocarcinogenesis common independent aetiology infection. Correspondence: