p53 gene mutation spectrum in hepatocellular carcinoma.

摘要: In order to clarify the significance of mutation p53 tumor suppressor gene in genesis and development human hepatocellular carcinoma (HCC) an aflatoxin B1 low-exposure area, spectrum, i.e., incidence, type, site, mutations was examined 169 tissue samples resected mainly from Japanese patients using single-strand conformation polymorphism analysis direct sequencing. Forty-nine tumors (29%) showed a (39 point 10 frameshifts). The comprised 18 transitions, only 4 which occurred at CpG sites, 21 transversions. Two evolutionarily conserved domains, IV V, contained 65% all codon 249 most frequent site (7/49). spectrum did not differ among HCCs relation type hepatitis virus infection, sex, age, background liver disease patients, size, or presence metastasis, but incidence were significantly associated with degree differentiation cancer cells. poorly differentiated HCC, (54%) clustered on domains whereas well moderately less (21%) equally distributed II V. Restriction fragment length revealed loss heterozygosity chromosome 17p 55 (69%) 80 informative cases 34 (95%) 36 mutation. Therefore, is suggested occur independently viral infection status preexisting preferentially association after another allele as late event HCC progression.