Interaction of the polyene antibiotics with lipid bilayer vesicles containing cholesterol.

摘要: Abstract The interaction of the polyene antibiotics, amphotericin B, nystatin and filipin with cholesterol-containing single bilayer lipid vesicles has been characterized using gel permeation chromatography proton magnetic resonance. All three antibiotics bind to at low concentrations without causing a large amount vesicle destruction. strength binding as determined by studies is greater for than nystatin. Nystatin B these induce rapid loss internal contents consistent pore formation. Filipin induces no leakage beyond that expected from partial destruction or general detergent action. At antibiotic levels above 1 : cholesterol ratios NMR results show all cause extensive onset this behavior, which appears be independent total concentration, indicates well defined stoichiometry. Despite fact required activity, spectra prior changes indicative an antibiotic-induced reversal restriction phosphatidylcholine mobility. contrast behavior in more extended bilayers discussed.