The acquisition of b-cell competence and diversity.

摘要: The in vitro splenic focus technique has been used to establish the characteristics of repertoire B cells adult BALB/c mice, its stimulation and development. Evidence is presented that yields an accurate estimate precursor cell frequency by providing monoclonal responses a constant portion (4%) transferred cells. Since monoclonally derived antibodies can be analyzed, it also possible determine isotype clonotype many resultant antibodies. spleen shown contain vast (over 10(7) clonotypes) primary cells, each represented from less than ten as 10(4) responsive Antigenic these highly specific apparently affinity dependent leads both antibody-forming clone formation population secondary Secondary though same clonotypes, differ several characteristics, including lower degree secificity antigenic stimulation. In addition, stimulated collaboration with allogeneic antigen-specific T yield clones producing antibody IgG1 isotope, whereas similarly produce only IgM Analyses neonatal indicate acquisition patterned process. Thus, clonotypes are present at birth, more acquired ordered pattern during first few weeks life. It concluded this process probably evolutionarily determined, since antigen-driven events appear play no role. However, developing exquisitely sensitive tolerance induction, negative selection may important role, particularly eliminating recognizing self-determinants for which species polymorphic.