Detection of association of IL1β, IL4R, and IL6 gene polymorphisms with cervical cancer in the Bangladeshi women by tetra-primer ARMS-PCR method

摘要: BACKGROUND Cervical cancer (CC) is the main cause of cancer-related deaths among women in developing countries. It second leading female malignancy Bangladesh terms incidence and mortality. Our present study aimed to investigate association IL1β (rs16944), IL4R (rs1801275), IL6 (rs1800797) gene polymorphisms with susceptibility cervical cancer. MATERIALS AND METHODS This case-control was conducted on 252 patients 228 healthy volunteers, using tetra-primer amplification refractory mutation system-polymerase chain reaction (ARMS-PCR). RESULTS In case rs16944 polymorphism, GG genotype (OR = 2.10, 95%CI = 1.24-3.56), dominant model (OR = 1.71, 95% CI = 1.11-2.63), recessive (OR = 1.54, CI = 1.01-2.35), G allele (OR = 1.30, CI = 1.005-1.68) were significantly associated increased risk. Among these, remained significant after Bonferroni correction (p < 0.017). For rs1801275 (OR = 2.66, CI = 1.49-4.75), (OR = 1.49, CI = 1.04-2.14), (OR = 2.45, CI = 1.40-4.27), (OR = 1.59, CI = 1.21-2.10) elevated risk but significance did not exist for correction. rs1800797 variant showed all genetic models including, AG (OR = 8.13, CI = 5.27-12.55), AA (OR = 9.86, CI = 2.76-35.21), (OR = 8.25, CI = 5.40-12.60), (OR = 4.41, CI = 1.25-15.56), A (OR = 4.99, CI = 3.49-7.13) significances consistent except model. Haplotyping analysis indicates that GAA (p = 5.15x10-5) GAG haplotypes (p = 4.72x10-9) decreased CC, whereas AAA (p = 3.89x10-9), AAG (p = 0.0003), AGA (p = 3.98x10-5) AGG (p = 0.002) CC. The mRNA level up-regulated, which poor prognosis silico. CONCLUSION results conclude (IL1β), (IL4R), (IL6) are Bangladeshi women.