A molecular signature for purified definitive endoderm guides differentiation and isolation of endoderm from mouse and human embryonic stem cells.

摘要: Embryonic definitive endoderm (DE) generates the epithelial compartment of vital organs such as liver, pancreas, and intestine. However, purification DE in mammals has not been achieved, limiting molecular “definition” endoderm, hindering our understanding development attempts to produce from sources embryonic stem (ES) cells. Here, we describe mouse using fluorescence-activated cell sorting (FACS) mice harboring a transgene encoding enhanced green fluorescent protein (eGFP) inserted into Sox17 locus, which is expressed endoderm. Comparison patterns signaling pathway activation native endoderm-like cells generated ES produced novel culture modifications that Sox17-eGFP+ progeny whose gene expression resembled more closely than achieved with standard methods. These studies also new FACS methods for purifying nontransgenic cultures. Parallel human SOX17-eGFP line allowed analysis differentiation vitro, leading an signature. This work should accelerate mechanisms regulating humans, guide further use tissue replacement.