作者: G Stingl , L A Gazze-Stingl , W Aberer , K Wolff
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摘要: Mice that are chronically exposed in vivo to ultraviolet B light (UV-B) display altered immunologic reactivity various antigenic stimuli. A possible mode of UV-B action is it exerts adverse effects on antigen-presenting cell function. Because the epidermis only tissue naturally subject UV exposure we investigated if murine epidermal cells (EC) could perform an antigen presentation function and, so, this be by irradiation. For purpose, T immune purified protein derivative tuberculin (PPD) and dinitrophenylated ovalbumin (DNP/sub 6/-OVA) from either BALB/c or C3H/He mice were incubated with syngeneic, semisyngeneic, allogeneic EC or, for control purposes, peritoneal exudate (PEC) had been pulse-exposed immunizing antigens as controls, left unpulsed, pulsed human serum albumin (HSA). After 4 days culture, proliferation was assessed /sup 3/H-thymidine incorporation. PPD- DNP/6-OVA pulsed, but not HSA-pulsed PEC, induced vigorous syngeneic allogeneic, cells. Pretreatment stimulator specific anti-Ia complement virtually abolished response, which indicated among EC, Ia-bearing Langerhans critical stimulators. Exposuremore » before after pulsing resulted a dose-dependent impairment antigen-specific proliferation; proliferative response administration 20 mJ/cm/sup 2/ UV-B. dose range employed did produce immediate lethal damage, premature death cultured toxic factors inhibitory proliferation.« less