Methods of Leukodepletion

摘要: This chapter reviews methods of production leukocyte-depleted blood components, comments concerning the procedures used in quality control these products and a discussion proved potential clinical benefits derived from transfusion lieu conventional white cell containing components. The advantages documented for use include reduced incidence nonhemolytic febrile reactions, minimized rate sensitization to HLA antigens accompanying immunological refractoriness platelet transfusions provision means provide “cytomegalovirus” safe other than traditional search seronegative donors. benefit leukodepleted curtailing induced HTLV-I transmission, avoiding immunomodulation which follows receipt decreasing infection tumor metastases may be statistically associated with allogeneic are interesting possibilities that require prospective study. During past three decades technologies have evolved primitive capable removing less 90% products’ native leukocytes those currently deplete −4 logl0 cells. These techniques sedimentation, centrifugation, washing, red freezing followed by deglycerolization, top bottom component preparation systems laboratory bedside filters. Although definition not leukocyte-depletion technique, ultraviolet irradiation concentrate has been recently shown hold reducing sensitization. As remove improve, so must qualify monitor products. At present lower standard components set at units no more 106 cells (U.S. standard, 5 x 106). Clinical studies change standards. Prototype filters now providing product as few 103 leukocytes. virtual elimination all could conceivably justify new applications, such lack elicit graft versus host disease.