Affinity chromatography of Nicotiana tabacum ribonucleases

摘要: Abstract The purification of tobacco ribonuclease by affinity chromatography is described. 5′-(4-amino-phenylphosphoryl)-guanosine 2′, (3′) phosphate, a ribonuelease inhibitor, has been synthesized and insolubilized onto agarose beads. resulting adsorbent binds some other plant ribonucleases strongly but reversibly at pH 5.4. bound enzyme can be eluted changing the or ionic strength eluting buffer, specific elution with substrate inhibitor. Binding not due to simple ion-exchange properties adsorbent.