In vivo analysis of plant promoters and transcription factors by agroinfiltration of tobacco leaves.

摘要: A convenient, Agrobacterium-mediated transient expression assay has been evaluated for rapid analysis of plant promoters and transcription factors in vivo. By simple infiltration Agrobacterium cells carrying appropriate plasmid constructs into tobacco leaves planta, reproducible assays could be conducted as little 2-3 days without using expensive equipment (e.g. biolistic gun or electroporation apparatus) complicated procedures preparation protoplasts). Biotic abiotic treatments applied to the intact examine their influence on promoter activity gene expression. Using this method, we have tested stress-responsive as-1 heat shock elements, yeast GAL4 transactivation system, two pathogenesis-related (PR) genes well a promoter. Through deletion analyses PR1a novel myb1 promoter, also successfully identified cis-regulatory regions these that are responsive salicylic acid treatment mosaic virus infection. Together, our results demonstrate is efficient method vivo factors.