A comparative genomic map for Caulanthus amplexicaulis and related species (Brassicaceae).

摘要: Adaptation to environment is the cornerstone of ecological genetics. The subject this study a wild relative sequenced and annotated model plant species, Arabidopsis thaliana. Caulanthus amplexicaulis var. barbarae lives on serpentine soils, known for high concentrations heavy metals low essential macronutrients, provides compelling example an organism's adaptation environment. We constructed F(2) linkage map, using cross nonserpentine sister taxon, C. amplexicaulis amplexicaulis. member highly diverse set taxa (within tribe Thelypodieae), described here as 'Streptanthoid Complex' that are adapted broad range environments, yet share common n = 14 chromosome number likely arose by recent radiation. map consists 97 polymorphic microsatellite markers, 40 exon-primed intron-crossing markers based A. thaliana exon sequences Brassica ESTs. covers 14 groups has total length 1513 cM. Both patterns marker segregation comparative indicate diploid organism with compact genome. All unexpectedly large (83%), had significant similarity genome, facilitating development genome map. As proof principle, we used identify candidate genes underlying differences in sepal colour between two parent taxa. demonstrate genomic tools developed will be portable throughout Streptanthoid Complex.