Immunohistochemical evaluation of p16INK4A, E-cadherin, and cyclin D1 expression in melanoma and Spitz tumors.

摘要: We evaluated the usefulness of immunohistochemical examination for E-cadherin, p16, and cyclin D1 in discriminating melanoma from Spitz tumors. Immunoperoxidase staining was performed on formalin-fixed tissue specimens 46 tumors 42 concurrent specimens. The percentages immunoreactive melanocytes epidermis dermis were estimated semiquantitatively. Qualitatively abnormal immunoreactivity patterns also tabulated. Dermal p16 best quantitative discriminator: decreased nuclear (<25% dermal melanocytes) 3-fold more likely than (P = .004). Loss both cytoplasmic 8-fold .01). Qualitative irregularities zonal distribution E-cadherin 2-fold higher .01), but these often focal or subtle. There no statistically significant difference immunoreactivity. In atypical tumors, frequency qualitative abnormalities intermediate between those ordinary nevi melanoma. Also, contrasting helpful determining Breslow thickness containing contiguous nevi.