Ancillary Techniques in Diagnosing Melanocytic Lesions

摘要: Currently ancillary techniques such as special stains, immunohistochemistry, and electron microscopy cannot replace the routine histologic slides; however, they can help to narrow differential adjuncts in diagnosing melanocytic lesions outlined this chapter. Immunohistochemistry utilizes antigen-antibody recognition detecting specific antigens within tissues. The sensitivity specificity of a given antibody, its pattern staining (nuclear, cytoplasmic, or membranous), background artifact must be considered interpretation. In addition, evaluation done relation internal control. setting poorly differentiated metastases, ultrastructural study is crucial classifying line differentiation.