Effects of Supplementing Aqueous Direct-Fed Microbials on In Vitro Fermentation and Fibrolytic Enzyme Activity in the Ruminant Nutrition

摘要: S. H. Lee* and I. J. Seo**Department of Animal Science, Korea National Agricultural College, RDA, Suwon 445-893, Korea*Woo Tech Co., Yongin 102-104, Korea**ABSTRACTThis study was conducted to determine effects supplementation levels aqueous direct-fed microbials(DFM; Bacillusspp.) TMR(exp. 1.) DFM addition under the various ratios starch andcellulose(exp. 2.) on ruminal fermentation fibrolytic enzyme activity. In experiment 1, fluidstaken from rumen-cannulated Holstein cows were incubated during 24 hr by using TMR as substrates.Aqueous applied at a rate 0, 0.025 0.05%, respectively. The pH 0.025% treatmentwas not significantly different that control 6 9 hr, but it lower (P<0.05)than 0.05% treatment.Concentrations ammonia-N VFAs affected supplementing DFM. A:Pratio treatment increased(P<0.05) ascompared with hr. Although overall activities significantlyaffected DFM, CMCase(carboxymethylcellulase) activity showed significantincrease(P<0.05) compared 6hr. However, xylanase treatmentsignificantly decreased(P<0.05) 12 due application There no significantdifference for invitro dry matter disappearance among treatments.In 2, fluids condition tocellulose (90:10, 70:30, 50:50, 30:70 10:90) or without DFM(0.025%). Ruminal wasCorresponding author:S. Lee, Department RDA,Suwon Korea. Tel:+82-11-9639-5037, Fax:+82-31-229-5055. E-mail:sh5037@hanmail.net