LC-MS/MS method for simultaneous determination of myclobutanil, hexaconazole, diniconazole, epoxiconazole and tetraconazole enantiomers in soil and earthworms

摘要: A new method using gel permeation chromatography (GPC) clean-up followed by liquid chromatography-tandem mass spectrometry (LC-MS/MS) was developed for the determination of myclobutanil, hexaconazole, diniconazole, epoxiconazole and tetraconazole enantiomers in soil earthworms. The earthworm samples were extracted with acetonitrile then purified a GPC procedure conducted on CO785 column cyclohexane ethyl acetate (50:50, V/V) as mobile phase at flow rate 4.7 mL/min. eluent between 7.5 min 15 min collected LC-MS/MS analysis. LC MS/MS conditions optimised. Formic acid (0.1%, added to water enhance MS responses. LODs 0.001–0.003 mg/kg. At three fortification levels 0.01–1.00 mg/kg, recoveries 81.2% 100.2% relative standard deviation below 14%. results experiment showed that hexaconzole diniconazole...