Glycine-Like Modulation of N-Methyl-d-Aspartate Receptors by a Monoclonal Antibody That Enhances Long-Term Potentiation

摘要: We have identified a monoclonal antibody, B6B21, that significantly elevates long-term potentiation when applied to CA1 pyramidal cell apical dendrites in rat hippocampal slices and characterized its binding N-methyl-D-aspartate-receptor complexes using extensively washed membranes. Five micrograms of affinity-purified B6B21 per 100 membranes gave two- threefold elevation N-[1-(2-thienyl)cyclohexyl]-3,4-[3H]piperidine ([3H]TCP) binding. When [3H]TCP was stimulated by the combined addition maximal concentrations glutamate, glycine, magnesium, no longer Like enhanced effect N-methyl-D-aspartate glutamate stimulating Moreover, reversed 7-chlorokynurenic acid inhibition binding, but it had on D-(-)-2-amino-5-phosphonovaleric acid. increased rate association dissociation [3H]TCP, equilibrium Glutamate, not however, B6B21-enhancement dissociation. at strychnine-insensitive glycine sites confirmed direct measurement [3H]glycine These results suggest binds directly receptors displays properties similar glycine.