Enhancement of Bacillus thuringiensis insecticidal activity by combining Cry1Ac and bi-functional toxin HWTX-XI from spider.
作者： Yunjun Sun , Zujiao Fu , Xiaohong He , Chunhua Yuan , Xuezhi Ding
DOI: 10.1016/J.JIP.2015.02.005
关键词:
摘要: Abstract In order to assess the potency of bi-functional HWTX-XI toxin from spider Ornithoctonus huwena in improving insecticidal activity Bacillus thuringiensis, a fusion gene cry1Ac and hwtx-XI was constructed expressed an acrystalliferous B. thuringiensis strain Cry-B. Western blot analysis microscopic observation revealed that recombinant could express 140-kDa Cry1Ac-HWTX-XI protein produce parasporal inclusions during sporulation. Bioassay using larvae Helicoverpa armigera Spodoptera exigua showed more toxic than control Cry1Ac protoxin, as by 95% lethal concentration. Our study indicated might be candidate for enhancing toxicity products.
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