Molecular cloning, bioinformatics analysis and functional characterization of HWTX-XI toxin superfamily from the spider Ornithoctonus huwena.
作者： Liping Jiang , Meichun Deng , Zhigui Duan , Xing Tang , Songping Liang
DOI: 10.1016/J.PEPTIDES.2014.01.001
关键词:
摘要: Spider venom contains a very valuable repertoire of natural resources to discover novel components for molecular diversity analyses and therapeutic applications. In this study, HWTX-XI toxins from the spider glands Ornithoctonus huwena which are Kunitz-type (KTTs) were directly cloned, analyzed functionally characterized. To date, superfamily consists 38 members deduced 121 high-quality expressed sequence tags, is largest KTT with significant mainly resulted cDNA tandem repeats as well focal hypermutation. Among them, HW11c40 HW11c50 may be intermediate variants between native Kunitz sub-Kunitz based on evolutionary analyses. order elucidate their biological activities, recombinant HW11c4, HW11c24, HW11c27 HW11c39 successfully expressed, further purified Both HW11c4 display inhibitory activities against trypsin, chymotrypsin kallikrein. Moreover, also an inhibitor relatively specific Kv1.1 channels. HW11c24 found inactive chymotrysin, kallikrein, thrombin ion These findings provide evidence toxin diversification useful templates serine protease inhibitors channel blockers development potentially clinical
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