Bacterial growth and phytoalexin elicitation in soybean cell suspension cultures inoculated with Pseudomonas syringae pathovars

摘要: Two commonly used media for the culturing of numerous plant tissues, 1-B5 and 1-B5C, were tested their ability to support bacterial growth. No significant growth strains representing four six major genera phytopathogenic bacteria occurred in liquid medium, contrast making highly suitable studies most bacteria—plant tissue culture interactions. Both compatible incompatible soybean leaf pathogen Pseudomonas syringae pv. glycinea plus bean pathogens P. phaseolicola , induced production phytoalexin glyceollin cv. Mandarin cell suspension cultures. Glyceollin was elicited without a concomitant hypersensitive response (HR). Induced by cultures declined as number transfers increased. There no expression resistance or cultured cells. This indicated by: (1) highest line Sb-2 after inoculation with strain, (2) greater than (3) an strain grew well strains. Of several which HR on leaves vivo only Y30 caused rapid decline viability Various bioassays attempt determine if Phytotoxin syringomycin responsible.