Decreased expression of lysophosphatidylcholine (16:0/OH) in high resolution imaging mass spectrometry independently predicts biochemical recurrence after surgical treatment for prostate cancer.

摘要: Background Human prostate cancers are highly heterogeneous, indicating a need for various novel biomarkers to predict their prognosis. Lipid metabolism affects numerous cellular processes, including cell growth, proliferation, differentiation, and motility. Direct profiling of lipids in tissue using high-resolution matrix-assisted laser desorption/ionization imaging mass spectrometry (HR-MALDI-IMS) may provide molecular details that supplement morphology. Methods Prostate samples were obtained from 31 patients, with localized cancer who underwent radical prostatectomy. The assessed by HR-MALDI-IMS positive mode, the molecules identified tandem (MS/MS). effect on specific antigen recurrence free survival after prostatectomy was determined Cox regression analysis Kaplan–Meier method. Results Thirteen found be expressed tissue, five being significantly lower than benign epithelium. MS/MS showed these [lysophosphatidylcholine (LPC)(16:0/OH)+H]+, [LPC(16:0/OH)+Na]+, [LPC(16:0/OH)+K]+, [LPC(16:0/OH)+matrix+H]+, [sphingomyelin (SM)(d18:1/16:0)+H]+. Reduced expression LPC(16:0/OH) an independent predictor biochemical prostatectomy. Conclusions HR-MALDI-IMS SM(d18:1/16:0) These differences phospholipids aggressiveness, new insights into lipid cancer. Prostate 75:1821–1830, 2015. © 2015 Wiley Periodicals, Inc.