Single-strand conformation polymorphisms in the hly gene and polymerase chain reaction analysis of a repeat region in the iap gene to identify and type Listeria monocytogenes.

摘要: Two novel methods that allow the powerful identification of Listeria monocytogenes by polymerase chain reaction (PCR) and simultaneous differentiation special electrophoresis formats are described. The first method involves a PCR-driven single-strand conformation polymorphism (SSCP-PCR) assay using portion noncoding region hlv gene. was evaluated with 120 genetically distinct L. strains either foodborne or clinical origin. Distribution listerial to at least 14 SSCP types observed. In respect panel strains, 39.7% were assigned type 3, 19% showed 5. Further, 1 found in 7.5% all 10 6.7%, 5.8% each for 6 7. 4, 9, 11 infrequently described 2.55%, 3.3%, 4.2%, respectively, isolates. At 0.85% represented 2, 13, 14, 1.7% displayed 8 12. second method, internal threonine-asparagine repeat p60 protein used setting up PCR-based parallel assay. Ten different (RTs), according sizes PCR products, Of 163 tested, 35.58% samples RT 1, 39.26% 3.68% 6.13% 4.29% 5, 2.45% 6, 5.52% 7, 0.61% 8, 1.83% 10. data suggest both simple Therefore, SSCP-PCR could represent pretyping assays before laborious reference typing applied.