Development of multilocus single strand conformation polymorphism (MLSSCP) analysis of virulence genes of Listeria monocytogenes and comparison with existing DNA typing methods

摘要: Development of rapid and simple typing methods is required for analyzing the distribution contamination routes food-borne pathogens. We established a method Listeria monocytogenes using MLSSCP (Multilocus Single Strand Conformation Polymorphism) analysis. Four virulence genes, hlyA, iap, actA inlB were amplified by PCR, digested with endonucleases applied to gels SSCP. As banding patterns have been shown reflect even single nucleotide difference, this has potential discriminatory power comparable that sequencing The 64 strains isolated from five meat processing plants divided into 18 groups MLSSCP. Additionally, clustering obtained showed strong correspondence phylogenetic lineages I II, was achieved much less expenditure in time cost than other methods, such as MLST. validity lineage classification confirmed PFGE, AFLP ribotyping results. This newly developed suitable when obtaining accurate results quickly simply crucial.