Comparison of ribotyping, arbitrarily primed PCR, and pulsed-field gel electrophoresis for molecular typing of Listeria monocytogenes.

摘要: Fifty-one clinical isolates of Listeria monocytogenes (15 from two outbreaks and 36 epidemiologically unrelated isolates) were typed by conventional serotyping, ribotyping (RT), pulsed-field gel electrophoresis (PFGE), arbitrarily primed PCR (AP-PCR). Serotyping was unable to distinguish between related strains L. monocytogenes. Each the three molecular methods showed excellent typeability reproducibility. Restriction with EcoRI PvuII gave 16 23 RT patterns, respectively. ApaI or SmaI generated 22 26 PFGE profiles, profiles easier interpret, 10 15 bands each, while had 20 each. AP-PCR different primers yielded 29 31 randomly amplified polymorphic DNA Strains same outbreak shared concordant patterns each methods. Of techniques evaluated, least discriminating could not outbreaks. The abilities differentiate comparable. However, more rapid perform. We conclude that either can be used clearly identify as being distinct one another. recommend at independent for typing in order improve its discriminatory power.