Immunomodulatory effects of aqueous birch pollen extracts and phytoprostanes on primary immune responses in vivo.

摘要: BACKGROUND: We recently demonstrated that pollen not only function as allergen carriers but also rich sources of bioactive lipids, such phytoprostanes, modulate human dendritic cell (DC) in a way results an enhanced T(H)2 polarization vitro. OBJECTIVE: Here we analyzed the immunomodulatory capacities Betula alba (white birch) aqueous extracts (Bet-APEs) and pollen-associated phytoprostanes murine system vitro vivo. METHODS: DC was by using BALB/c bone marrow-derived DCs. T-cell responses were with DO11.10 peptide 323-339 from chicken ovalbumin (OVA)-specific CD4 T cells responder cells. For vivo studies, OVA-specific adoptively transferred into mice. Twenty-four hours later, mice challenged means intranasal application OVA absence or presence Bet-APEs phytoprostanes. Polarization draining lymph node RESULTS: In E(1)-phytoprostanes dose-dependently inhibited LPS-induced IL-12p70 addition, induced Similarly, instillation vivo, together antigen, lead to increased IL-4, IL-5, IL-13 secretion decreased IFN-gamma antigen-specific nodes. contrast, E1- F1-phytoprostanes downregulated both T(H)1 cytokine production CONCLUSION: Pollen release water-soluble factors display T(H)2-polarizing independently E(1)- F(1)-phytoprostanes. CLINICAL IMPLICATIONS: Identification underlying mechanisms might open new approaches for pharmacologic intervention.