The Ferritin-Like Dps Protein Is Required for Salmonella enterica Serovar Typhimurium Oxidative Stress Resistance and Virulence
作者： Thomas A. Halsey , Andrés Vazquez-Torres , Daniel J. Gravdahl , Ferric C. Fang , Stephen J. Libby
DOI: 10.1128/IAI.72.2.1155-1158.2004
关键词:
摘要: Resistance to phagocyte-derived reactive oxygen species is essential for Salmonella enterica serovar Typhimurium pathogenesis. can enhance its resistance oxidants through the induction of specific genetic pathways controlled by SoxRS, OxyR, sigma(S), sigma(E), SlyA, and RecA. These regulons be found in a wide variety pathogenic environmental bacteria, suggesting that evolutionarily conserved mechanisms defend against oxidative stress both endogenously generated aerobic respiration exogenously produced host phagocytic cells. Dps, ferritin-like protein many eubacterial archaebacterial species, appears protect cells from sequestering iron limiting Fenton-catalyzed oxyradical formation. In Escherichia coli some other bacterial Dps has been shown accumulate during stationary phase sigma(S)-dependent fashion, bind nonspecifically DNA, form crystalline structure compacts protects chromatin damage. present study, we provide evidence iron-dependent killing hydrogen peroxide, promotes survival murine macrophages, enhances virulence. Reduced numbers dps mutant bacteria livers spleens infected mice are consistent with role protecting encountered infection.
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