The Effects of Exercise and a Low-Fat Diet on Monocyte TLR Expression and Disease Risk in Mice

摘要: Excess adiposity increases systemic inflammation, which is implicated in various diseases. Physical activity a common treatment to reverse weight gain and increased disease risk; however, little evidence available determine if forced or voluntary exercise more effective mice. Such information has implications for the refinement of human interventions. PURPOSE: To 8-weeks combined with low-fat feeding reverses changes risk, monocyte concentration TLR2/TLR4 expression. METHODS: For 12-months, 24 CD-1 mice underwent pre-treatment, consuming either (10% kcal from fat, N=6) high-fat (60% N=18) diet ad libitum. High-fat fed were randomly assigned one three groups (N=6/group): V-EX (low-fat chow, access running wheel 5 d/week), F-EX treadmill at 22 m/min, 60min/d, SD no exercise). Mice pre-treated chow served as controls (CN, N=6). Measurements made on weekly saphenous vein blood samples using 3-color flow cytometry. Blood glucose cholesterol analyzed analyzer. IPGTT was performed baseline week 8 area under curve. RESULTS: All lost significant body over (P<0.001). ran 4.4x than There effects cholesterol. CN had 26% higher levels (P=0.009). On average, there 59% decrease AUC decreased 37% CN. At 8, 6x 1 (P=0.002). Specifically, greater both (49%) (59%). Cell-surface TLR2 expression significantly 6 (55%) (23%) relative baseline. Monocyte cell-surface TLR4 CONCLUSIONS: Combining caused loss alone. Overall, insulin resistance glucose, suggesting may have been type II diabetes risk. Lack difference TLR2/4 suggest intervention not long enough elicit inflammation following long-term feeding. More research needed understand time course these changes. This study useful understanding what occurs during directly comparing exercise.