Conditional immortalization of growth factor-responsive cardiac endothelial cells from H-2Kb-tsA58 mice

摘要: Although mouse endothelial cells (EC) may advance our understanding of function, primary EC remain difficult to isolate. We have established a murine cardiac cell line (MCEC-1) from transgenic mice harboring temperature-sensitive simian virus 40 large TAg gene (tsA58 TAg) under H-2K(b) class I promoter control. MCEC-1 were characterized by their ability form tubes, Griffonia simplicifolia isolectin B4 binding, and CD31, intercellular adhesion molecule (ICAM)-2, endoglin expression. proliferated rapidly permissive conditions [33 degrees C with interferon (IFN)-gamma], where the T antigen is active transcription activated presence IFN-gamma, whereas nonpermissive (38 without IFN-gamma) proliferation was reduced 30-fold showed enhanced in response growth factors. Expression E- P-selectin, ICAM-1, vascular molecule-1 upregulated tumor necrosis factor-alpha interleukin-1 beta, cells, contrast EC, amenable transfection lipofection. This novel will allow further study role endothelium cardiovascular disease. Moreover, this technique be readily obtained genetically modified backcrossed H-2K(b)-tsA58 mice.