Apoptosis-related proteins are potential markers of neonatal hypoxic-ischemic encephalopathy (HIE) injury.

摘要: Abstract Neonatal hypoxic–ischemic encephalopathy (HIE) causes high mortality and long-term morbidity rates. The magnitude of the neuronal damage depends on duration severity initial insult combined with deleterious effects reperfusion apoptosis. Currently, a diagnosis HIE is based largely neurological histological findings. Therefore, aim this study was to identify apoptosis-related proteins that might serve as potential markers injury. As an step toward reaching objective, we analyzed changes in protein levels vitro model hypoxia using antibody arrays, have identified expression level two involved apoptosis, Smac-DIABLO cathepsin D. We obtained brain sections from eight neonatal patients performed staining, TUNEL assays D immunolocalization. Our results revealed number TUNEL-positive cells, including neurons, astrocytes ependymal various regions were analyzed. Interestingly, many areas positive for staining did not appear be damaged evaluation. In addition, immunostaining, found had same regional distribution pattern. Taken together, these findings indicate could injured detectable observations alone.