Two region-dependent pathways of eosinophilic neuronal death after transient cerebral ischemia

摘要: Various types of eosinophilic neurons (ENs) are found in the post-ischemic brain. We examined temporal profile ENs core and peripheral regions ischemic cortex, analyzed relationship to expression various cell death-related factors. Unilateral forebrain ischemia was induced Mongolian gerbils by transient common carotid artery occlusions, brains from 3 h 2 weeks post-ischemia were prepared for morphometric immunohistochemical analysis ENs. with minimally abnormal nuclei swollen bodies appeared at 12 periphery. In both locations multiple factors including calcium, micro-calpain, cathepsin D, 78 kDa glucose-regulated protein (GRP78) ubiquitin activated. core, pyknosis irregularly atrophic cytoplasm peaked h, which associated significant increases staining calcium micro-calpain. scant 4 days positive TUNEL staining. periphery, had slightly sequentially developed pyknosis, karyorrhexis karyolysis over 1 week. These cells All EN negative caspase 3. There may be two region-dependent pathways changes brain: cytoplasmic shrinkage nuclear disintegration This difference coordinates different activation patterns