Direct evidence for involvement of a guanine nucleotide-binding protein in chemotactic peptide-stimulated formation of inositol bisphosphate and trisphosphate in differentiated human leukemic (HL-60) cells. Reconstitution with Gi or Go of the plasma membranes ADP-ribosylated by pertussis toxin.
作者： A Kikuchi , O Kozawa , K Kaibuchi , T Katada , M Ui
DOI: 10.1016/S0021-9258(18)67279-5
关键词:
摘要: fMet-Leu-Phe (fMLP) stimulated the formation of inositol bis- and trisphosphate in [3H]inositol-labeled plasma membranes from human leukemic (HL-60) cells differentiated to neutrophil-like by dibutyryl cyclic AMP. The stimulatory effect fMLP was completely dependent on simultaneous presence GTP Ca2+. fMLP-stimulated phosphorylated inositols markedly reduced prior ADP-ribosylation with pertussis toxin. This toxin ADP-ribosylated a Mr approximately 40,000 protein, presumably alpha subunit Gi and/or Go, membranes. Reconstitution or Go purified rat brain restored inositols. efficiency this capacity roughly equal. beforehand inactive reconstitution. These results indicate that both have potency couple functionally receptor phospholipase C-mediated polyphosphoinositide hydrolysis suggest may be involved mechanism signal transduction reaction HL-60 cells.
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