Interaction of cellulase with sodium dodecyl sulfate at critical micelle concentration level

摘要: The interactions between Trichoderma reesei cellulase and an anionic surfactant, sodium dodecyl sulfate (SDS), at critical micelle concentration level have been investigated using isothermal titration calorimetry, fluorescence spectroscopy, circular dichroism. SDS micelles dual with cellulase: electrostatic first then hydrophobic interactions. When the of is smaller than 45.0mM, cause a partial loss in hydrolytic activity together steep decrease alpha-helical content cellulase. With further increasing SDS, however, re-formation structure recovery induced by are observed. Taken together, these results indicate that exert effects on through binding as both denaturant reagent.