Synthesis of Fucosyl-N-Acetylglucosamine Disaccharides by Transfucosylation Using α-l-Fucosidases from Lactobacillus casei

作者: Jesús Rodríguez-Díaz , Rodrigo J. Carbajo , Antonio Pineda-Lucena , Vicente Monedero , María J. Yebra

DOI: 10.1128/AEM.00229-13

关键词:

摘要: AlfB and AlfC α-l-fucosidases from Lactobacillus casei were used in transglycosylation reactions, they showed high efficiency synthesizing fucosyldisaccharides. activities exclusively produced fucosyl-α-1,3-N-acetylglucosamine fucosyl-α-1,6-N-acetylglucosamine, respectively. The reaction kinetics that can convert 23% p-nitrophenyl-α-l-fucopyranoside into at up to 56% fucosyl-α-1,6-N-acetylglucosamine.

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