α-l-Fucosidases: Exoglycosidases with Unusual Transglycosylation Properties

摘要: α-l-Fucosidases (EC 3.2.1.51), the only members of CAZy family GH-29, are widespread glycosidases involved in many biological processes including inflammation, metastasis, and lysosomal storage disease fucosidosis. Despite their significance, information concerning mechanism α-l-fucosidases has recently become available. In an attempt to obtain further data mechanism, we have investigated hydrolytic transglycosylation properties a canine mollusk (Pecten maximus) α-l-fucosidase. Our results show that, despite evolutionary distance between these two species, both enzymes similar hydrolysis properties. Surprisingly, found starting from monosaccharides, exoglycosidases were able catalyze efficiently synthesis highly branched fuco-oligosaccharides as large tetrasaccharides, unique feature for wild-type exoglycosidase. The structural analysis compounds formed revealed that ...